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CARS and SRS Raman Microscopy

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CARS and SRS Raman Microscopy

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Raman microscopy is a technique that enables label-free chemical imaging. It is based on the Raman scattering effect of molecules that was discovered by C.V. Raman in the early 1930s. When light with a particular wavelength or photon energy is incident on a molecule, it can be inelastically scattered. This means that part of the energy is absorbed by the molecule and the scattered photon has a lower energy than the incident photon (see Figure 1). The fraction of the energy absorbed depends on the vibrational frequencies of the molecule. All molecules have specific Raman frequencies, typically given in wavenumbers, which span from 100 cm-1 to 3500 cm-1. A molecule's Raman spectrum is highly dependent on its chemical structure but mostly unaffected by the local environment. Therefore, Raman spectroscopy is not only specific but also robust to environmental variability.

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