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The Benefits of Ultrashort Pulses in Two-Photon Microscopy

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The Benefits of Ultrashort Pulses in Two-Photon Microscopy

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  • Author: Andrew Wells
It has been shown in numerous papers, that the use of ultra-short (<10 fs) pulses has important benefits for two-photon microscopy. With short pulses, the instantaneous peak power for a given average output is much higher. This results in reduced scattering allowing deeper imaging, higher fluorescent efficiency (figure 1) and improved image contrast, all without increasing the photo-damage inflicted on the sample

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