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Single-molecule Localisation Microscopy (SLMS)

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Single-molecule Localisation Microscopy (SLMS)

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The fundamental principle of all SMLM implementations is the random photoactivation and subsequent localization of individual fluorescence emitters by detecting their photon emission at a certain position and time in the complete absence of emission from neighboring emitters. The centroid of the diffraction-limited distribution of photons is a measure of the location of the emitter and is far better defined than the photon distribution itself.

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