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Counting Peripheral Blood Mononuclear Cells Using the Corning® Cell Counter — A Brightfield Alternative to Manual Counting

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Counting Peripheral Blood Mononuclear Cells Using the Corning® Cell Counter — A Brightfield Alternative to Manual Counting

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  • Author: Jeff Partridge
Whole blood is collected from a donor and PBMCs are separated and saved while the remaining blood cells and components are returned to the body (apheresis)1. This process yields large amounts of PBMC which are suitable for further experimentation or study. PMBCs are smaller in size than many mammalian cell types, and depending on the sample, may contain erythrocyte (red blood cell, RBC) contamination. It is recommended that RBCs are removed by centrifugal separation or lysis prior to counting with the Corning Cell Counter

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