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Scaling-up cells in Falcon® Cell Culture Multi-Flasks

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Scaling-up cells in Falcon® Cell Culture Multi-Flasks

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  • Author: Suparna Sanyal, Elizabeth Abraham, Katie Slater, Amy Cai, Bill Lacey, Christel Hartshorn, Paula Flaherty, Brian McHugh, Susan Qian
A growing number of cell-based applications require a large number of cells. These applications typically commence in a small scale during development phase and are subsequently scaled-up once an assay or application is validated. Use of single-layer T-flasks may be adequate during development, but they can become cumbersome, laborious and inefficient when a large number of cells is required. For efficient scale-up of adherent mammalian cell cultures, it is important to use vessels designed to generate homogeneous cells on a consistent basis while retaining original cellular characteristics such as morphology, growth and function. Vessel design is equally important for optimal cell or product recovery.

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