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Raman Light Sheet Microscopy: A Fast Micro-Spectroscopy Imaging Technique

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Raman Light Sheet Microscopy: A Fast Micro-Spectroscopy Imaging Technique

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  • Author: Israel Rocha-Mendoza, Jacob Licea-Rodriguez, Monica Marro, Omar E. Olarte, Emilio J. Gualda, and, Pablo Loza-Alvarez
Raman spectroscopy provides information about the chemical composition of materials, cells and tissues. The Raman process is induced by scattering of the excitation light with the vibration of the molecules contained in the sample. This provides an intrinsic label-free contrast mechanism from the sample that is rich in biochemical content. Taking 2D information of the molecular distribution that conforms the sample is thus, very attractive to the biomedical science community. However, the efficiency of the Raman scattering process is very low and, therefore, long acquisition times (that goes from few minutes to several hours) are required to obtain a single 2D image.

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