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Multi-Line Lasers Simplify Fluorescence Microscopy

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Multi-Line Lasers Simplify Fluorescence Microscopy

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Fluorescence microscopy instrumentation relies on illumination sources to excite fluorophores. Common illumination sources are LEDs, super-continuum white-light sources, or single-wavelength lasers. Lasers are primarily used for high-resolution and high-throughput imaging techniques, and each wavelength excites a different set of fluorophores. In order to efficiently excite multiple fluorophores, it is necessary to use many single-wavelength lasers in one instrument or experiment. This strengthens the content and quality of results. Along with the advantage of activating more fluorophores comes the challenge of integrating each of the individual wavelengths required.

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